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KMID : 0388220110180020085
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Journal of the Korean Rheumatism Association
2011 Volume.18 No. 2 p.85 ~ p.93
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Inhibition of the IL-1?-induced Expression of Matrix Metalloproteinases by Controlled Release of IL-1 Receptor Antagonist Using Injectable and Thermo-reversible Gels in Human Osteoarthritis Chondrocytes
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Jun Hey-Jung
Kim Jang-Kyoung
Kim Tae-Hwan
Na Young-In
Choi Choong-Hyeok
Kim Yong-Hee
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Abstract
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Objective: IL-1? is involved in the degradation of articular cartilage in various arthritides, including osteoarthritis (OA). Competitive inhibition of IL-1? by IL-1 receptor antagonists (IL-1Ra) may represent a pathogenesis-based strategy for inhibiting degradation of the cartilage matrix. We investigated the hypothesis that controlled release of IL-1Ra using injectable, thermoreversible and complex coacervate combination gels as drug delivery systems might reduce matrix degradation in OA.
Methods: Thermoreversible combination gels that can be injected into joints were formed in aqueous solution by making a complex coacervate with recombinant human IL-1Ra (anakinra) and cationic macromolecules, and this was followed by co-formulation with methylcellulose as a negative thermosensitive polysaccharide. Gels containing anakinra were positioned in the upper insert of a transwell system and human OA chondrocytes were placed in the lower compartment and then they were stimulated with IL-1?. The expression of matrix metalloproteinases (MMPs) was examined by performing real time PCR and ELISA.
Results: Complex coacervation between anakinra and protamine was successfully completed. IL-1Ra was released from the gels in a sustained release pattern for extended periods with minimal initial bursts. IL-1? markedly enhanced the expression of MMP. The IL-1Ra released from the gels significantly inhibited the IL-1?-induced MMP expression in the chondrocytes.
Conslusion: We developed and optimized a novel injectable and thermoreversible gel system for the controlled release of IL-1Ra, and this drug delivery system effectively inhibited the IL-1?-induced MMP expression of chondrocytes in a transwell system. Intra-articular local delivery of injectable and thermoreversible gels containing IL-1Ra into knees has the potential to provide prolonged therapy based on the pathophysiology of knee OA.
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KEYWORD
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Drug delivery system, Interleukin-1, Interleukin-1 receptor antagonist, Methylcellulose gel, Osteoarthritis
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